منابع مشابه
Assembling yeast complexes
he machine that is a cell is more than the sum of its protein parts. A large step toward understanding how those parts are so effectively put together has been taken by Patrick Aloy, Rob Russell (EMBL, Heidelberg, Germany), and colleagues. Their work has identified ف 100 yeast protein complexes and predicted interactions within and among many of them. " We were working on a large scale to mod...
متن کاملLuminescent gels by self-assembling platinum complexes.
A platinum complex bearing a tetraethylene glycol chain has been designed and its self-assembly properties investigated. In solution, only a yellow phosphorescence of the aggregated species is observed. The complex gives luminescent gels of different colours with DCM and DMF, reaching up to 60% photoluminescence quantum yield.
متن کاملEvaluation of a symmetry-based strategy for assembling protein complexes.
We evaluate a strategy for assembling proteins into large cage-like structures, based on the symmetry associated with the native protein's quaternary structure. Using a trimeric protein, KDPG aldolase, as a building block, two fusion proteins were designed that could assemble together upon mixing. The fusion proteins, designated A-(+) and A-(-), comprise the aldolase domain, a short, flexible s...
متن کاملOptimized Affinity Capture of Yeast Protein Complexes.
Here, we describe an affinity isolation protocol. It uses cryomilled yeast cell powder for producing cell extracts and antibody-conjugated paramagnetic beads for affinity capture. Guidelines for determining the optimal extraction solvent composition are provided. Captured proteins are eluted in a denaturing solvent (sodium dodecyl sulfate polyacrylamide gel electrophoresis sample buffer) for ge...
متن کاملAn Essential Role for DYF-11/MIP-T3 in Assembling Functional Intraflagellar Transport Complexes
MIP-T3 is a human protein found previously to associate with microtubules and the kinesin-interacting neuronal protein DISC1 (Disrupted-in-Schizophrenia 1), but whose cellular function(s) remains unknown. Here we demonstrate that the C. elegans MIP-T3 ortholog DYF-11 is an intraflagellar transport (IFT) protein that plays a critical role in assembling functional kinesin motor-IFT particle compl...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2004
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb1651rr4